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Agentic-SpliceAI Documentation

Central documentation hub for Agentic-SpliceAI — Context-Aware Novel Isoform Discovery for Drug Target Identification.


📁 Topic Index

Getting Started

Document Description
QUICKSTART.md Get running in 5 minutes
SETUP.md Full environment setup
getting_started/RUNPODS_SETUP.md Cloud GPU (RunPods) workflow
getting_started/LATEX_SETUP.md LaTeX / PDF generation setup

Architecture

Document Description
architecture/README.md System architecture overview
architecture/STRUCTURE.md Full project directory structure
architecture/PACKAGE_ORGANIZATION.md Package organization guide

System Design

Document Description
system_design/README.md System design overview
system_design/resource_management.md Genomic resource registry
system_design/output_management.md Output & artifact management
system_design/configuration_system.md Configuration (Pydantic)
system_design/base_layer_architecture.md Base layer architecture

Base Layer

Document Description
base_layer/DATA_PREPARATION_CLI.md Data preparation CLI
base_layer/PROCESSING_ARCHITECTURE.md Chunking & processing architecture
base_layer/POSITION_COORDINATE_SYSTEMS.md Position / coordinate system reference

Multimodal Feature Engineering

Document Description
multimodal_feature_engineering/feature_catalog.md Complete 9-modality, 100-column feature reference
Epigenetic marks tutorial H3K36me3/H3K4me3 ChIP-seq modality
RBP eCLIP tutorial ENCODE RBP binding modality
Chromatin accessibility tutorial ENCODE ATAC-seq modality

Meta Layer

Document Description
meta_layer/README.md Meta layer overview
meta_layer/ARCHITECTURE.md Foundation-Adaptor architecture
M1-M4 model variants Four meta-layer model variants for splice prediction
meta_layer/methods/README.md Methods overview
meta_layer/methods/ROADMAP.md Development roadmap
meta_layer/methods/PAIRED_DELTA_PREDICTION.md Paired delta prediction method
meta_layer/methods/VALIDATED_DELTA_PREDICTION.md Validated delta prediction method
meta_layer/experiments/README.md Experiments index

Compute Resources

Document Description
compute/README.md Compute resource management overview
compute/remote_training_options.md Modal vs SkyPilot vs RunPods comparison
getting_started/RUNPODS_SETUP.md RunPods first-time setup guide

Foundation Models

Document Description
foundation_models/README.md Foundation models overview
foundation_models/evo2/junction_support_labels.md Evo2 junction support labels
foundation_models/training/deepspeed_training.md DeepSpeed training guide

Applications & Use Cases

Document Description
applications/README.md Domain-specific application workflows
isoform_discovery/README.md Novel isoform discovery overview
variant_analysis/README.md Variant analysis overview

Agentic Workflows

Document Description
agency/README.md Agentic workflow overview
agency/AGENTIC_MEMORY_TUTORIAL.md Memory system tutorial
agency/MEMORY_PATTERNS.md Memory design patterns

Tutorials & Workflows

Document Description
tutorials/README.md Tutorials overview
tutorials/SPLICE_PREDICTION_GUIDE.md Splice site prediction guide
workflows/README.md Analysis workflows

Domain Knowledge

Document Description
biology/README.md Splice site biology background
bioinformatics/README.md Bioinformatics methods

API Reference

Document Description
api/README.md REST & Python API reference
api/ENDPOINTS.md API endpoints, configuration, and data format

Project

Document Description
ROADMAP.md Development roadmap (Phases 1-10+)

📖 Documentation Conventions

  • docs/ — Official, centralized documentation (mkdocs + Obsidian). All new tutorial and reference notes go here.
  • Package-level docs/ (e.g., src/agentic_spliceai/docs/) — Temporary working notes close to the code. Promoted to docs/<topic>/ when mature.
  • dev/ — Private development notes, session logs, refactoring plans. Not in mkdocs or Obsidian.

🎯 Find What You Need

I want to... Go to
Get running fast QUICKSTART.md
Understand the pipeline architecture/README.md
Learn about the base layer base_layer/
Understand meta-learning meta_layer/
Work with foundation models foundation_models/
Discover novel isoforms isoform_discovery/README.md
Use agentic validation agency/README.md
Follow a tutorial tutorials/SPLICE_PREDICTION_GUIDE.md
Read splice biology biology/README.md
Use the API api/ENDPOINTS.md
Explore multimodal features multimodal_feature_engineering/feature_catalog.md
See the roadmap ROADMAP.md
Find application workflows applications/README.md

Last Updated: March 2026